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. Author manuscript; available in PMC: 2011 Apr 14.
Published in final edited form as: Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5509. doi: 10.1101/pdb.prot5509
Reagent Quantity (for 1 L) Final concentration
Na2HPO4 11.9 g 84.1 mM
NaH2PO4 (anhydrous) 2.23 g 18.6 mM
NaCl 102.2 g 1.75 M
Bring the volume to 1 L with distilled water. Adjust the pH to 7.4 and autoclave before
use. Prepare the working strength solution (1×, simply referred to as PBS in the
protocol) by diluting 1:10 with sterile dH20. Both 1× and 10× PBS are stored at room
temperature.