Table 3.

Purification timeline

Step	Time for completion		Stop point
	Preparation	Incubation
Growing protein ELP fusions
Day 1:Starter culture *an extra day for transformation may be required prior to this step	< 1 h	O/N	-
Day 2: Expression culture	< 1 h	24 h (8h for induction protocol)	-
Day 3: Cell cultivation	1 h	-	Store at -80° C
Lysing cells & ITC rounds
Day 4: Sonication	1 h	-	Must continue to purification
Day 4 or 5: ITC rounds (each)	2 h	-	After hot spin resuspension or cold spin store at -20° C
Day 4 or 5: Sample analysis	1 h	-	-
Protease cleavage and ELP tag removal (Day 5 or 6)	2 h	16 h	Cleaved product can be stored at -20°C