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. Author manuscript; available in PMC: 2011 Apr 14.
Published in final edited form as: Prog Neuropsychopharmacol Biol Psychiatry. 2004 Nov 4;29(1):7–13. doi: 10.1016/j.pnpbp.2004.08.004

Fig. 1.

Fig. 1

Specific concentration curves for 5-HT uptake and paroxetine binding to platelet membranes. (A) 5-HT uptake was performed as described in Methods in the absence and presence of 50 μM fluoxetine. Specific 5-HT uptake was determined by subtracting nonspecific uptake from total uptake. (B) [3H]paroxetine binding was performed as described in Methods in the absence and presence of fluoxetine. Specific [3H]paroxetine binding was determined by subtracting nonspecific binding from total binding. Km and Vmax values for 5-HT uptake and Kd and Bmax values for paroxetine binding were calculated using the one-site binding equation in Prism 4 software.