Fig. 3.

Analysis of porphyrin pigments accumulating in chl27-as lines after feeding with ALA in the dark. (A) Room-temperature fluorescence emission spectra of acetone extracts of chlorotic (yellow) leaves from an antisense line (chl27-as2.38.10) and green leaves from WT plants after overnight incubation with ALA compared to purified standards. (B) HPLC traces of the extracted pigment mixtures relative to known standards. The eluate was monitored at 420 nm for optimal detection of MgPMME and MgP (i, iii, v, and vii) or 440 nm for detection of Pchlide (ii, iv, and vi). (Inset) Traces show spectral absorbance of the major eluate peak, measured from 350 to 595 nm, with the wavelength of maximum absorbance indicated.