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. 2003 Dec 12;100(26):16119–16124. doi: 10.1073/pnas.2136793100

Fig. 4.

Fig. 4.

Suborganellar localization of CHL27. (A) Analysis of Arabidopsis chloroplast fractions. E, envelope; T, thylakoid; S, stroma. (Left) Fractions analyzed after SDS/PAGE by Coomassie blue staining. (Right) Immunoblot detection of CHL27 and OEP21. (B) Distribution of CHL27 between envelope fractions from spinach chloroplasts. Envelope membrane fractions were enriched in inner-envelope membrane (IM) or outer-envelope membrane (OM). T0,T100, and T600, envelope fractions were obtained from chloroplasts treated without thermolysin or with 100 or 600 μg/ml thermolysin, respectively. (Left) Coomassie blue staining. (Right) Immunodetection of CHL27, E37, an inner-envelope membrane protein, and E24, an outer-envelope membrane protein. Each lane was loaded with 20 μg of protein. The membrane was incubated with anti-CHL27 (1:1,000), anti-E37 (1:10,000), and anti-E24 (1:5,000).