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. 2011 Mar 30;18(2):107–115. doi: 10.1093/dnares/dsr003

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© The Author 2011. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Representative genotyping and mapping of eight SSR marker loci on soybean chromosome 5 (LG A1). (A) Multiplex PCR analysis of the eight SSR marker loci with primer sets labelled with four different fluorophores: 6-FAM (Sat_137 and Satt717), VIC (GMES3790 and Satt599), NED (GMES2497 and Satt545), and PET (Sat_344 and Sat_384). The separation and detection of PCR products corresponding to alleles of the eight marker loci with a fluorescence-based sequencer and GeneMapper 4.0 software are shown. (B) Genetic linkage map constructed with MAPMAKER/EXP 3.0b for the polymorphic SSR marker loci and the 94 F₂ individuals of the JI population derived from a cross between JP-36121 (wild soybean accession) and Ibarakimame 7 (landrace).