Skip to main content
. Author manuscript; available in PMC: 2011 Sep 17.
Published in final edited form as: Nature. 2011 Feb 6;471(7338):325–330. doi: 10.1038/nature09830

Figure 2. Alu RNA accumulation in GA triggered by DICER reduction.

Figure 2

a, b, dsRNA immunolocalized (blue) in RPE (a, b) and sub-RPE deposits (drusen; b) in human GA. c, d, No staining with isotype antibody in GA RPE (c) and with anti-dsRNA antibody in control eye (d). Scale bars, (a–d), 10 µm. n=10 (a–d) e, PCR amplification of immunoprecipitated dsRNA yielded amplicons with homology to Alu in GA RPE but not normal RPE. Water control (−) showed no amplification and recombinant dsRNA (+) showed predicted amplicon. f, Increased Alu RNA in GA RPE compared to control (n=7). P < 0.05 by Student t test. No significant difference in Alu RNA in neural retina. Values normalized to abundance in normal eyes.