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. Author manuscript; available in PMC: 2011 Sep 17.
Published in final edited form as: Nature. 2011 Feb 6;471(7338):325–330. doi: 10.1038/nature09830

Figure 3. DICER1 degrades Alu RNA.

Figure 3

a, DICER1 antisense (as) increased Alu RNA in human RPE cells. b, c, Ad-Cre, but not Ad-GFP, increased B1 and B2 RNAs in Dicer1f/f mouse RPE cells in nucleus (b) and cytoplasm (c). d, DICER1 as upregulated Alu RNA in human RPE cell nucleus (Nuc) and cytoplasm (Cyt). e, Agarose gel electrophoresis shows recombinant DICER1 (+), but not heat denatured DICER1, degrades Alu RNA isolated and cloned from human GA RPE. Image representative of 6 experiments. f, Alu RNA in human RPE cells upregulated by plasmid coding for Alu (pAlu) vs. pNull or no treatment (no Tx) at 24 h reduced by pDICER1. * P < 0.05. n=4–8 (a–d, f). Values normalized to control as-treated (for Alu) or Ad-GFP-infected cells (for B elements).