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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Cancer Res. 2011 Feb 15;71(5):1978–1988. doi: 10.1158/0008-5472.CAN-10-2512

Figure 1. Identification of a SRF-dependent androgen-responsive expression profile.

Figure 1

LNCaP cells were transfected with siRNA targeting SRF or control siRNA (c) as described. RNA was isolated, column purified and processed for hybridization to Human Genome Affymetrix U133 Plus 2.0 arrays as described. Microarray raw data was analyzed and heatmaps were generated as described. 113 unique androgen-induced genes (131 probe sets) (left panel) and 45 unique androgen-repressed genes (47 probe sets) (right panel) were identified. Each row on the heatmap represents a probe set and each column represents an individual replicate. Gene expression on each probe set was standardized on the mean of samples where red color is higher than the mean and green color is lower than the mean (A). The 158 gene signature which relies on SRF for full androgen regulation represents 5.5 percent of all genes which are at least 2-fold regulated by androgen treatment in control-transfected condition (B).