. Author manuscript; available in PMC: 2012 Mar 23.

Published in final edited form as: J Am Chem Soc. 2011 Feb 25;133(11):4101–4114. doi: 10.1021/ja110907m

Table 3.

Apparent Metal Binding Constants of ZPP1 and 6-Functionalized Derivatives.

	K′₁^a,^b (M⁻¹)	K′₂^a,^b (M⁻¹)
ZPP1	2.6(2)×10⁹	6.4(2)×10⁷
ZPP1(6-CO₂H)	1.9(1)×10⁹	5.5(3)×10⁷
ZPP1(6-CO₂Et)	3.1(3)×10⁹	6.9(1)×10⁷

Measurements performed in aqueous buffer at pH 7.0, 50 mM PIPES, 100 mM KCl.

K′ represents the conditional equilibrium constant corresponding to stepwise zinc binding events at fixed pH. Numbers in parenthesis represent standard deviations in the last significant digits, estimated from the analysis of three separate runs.