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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Neuroendocrinol. 2011 Feb;23(2):168–176. doi: 10.1111/j.1365-2826.2010.02086.x

Figure 3.

Figure 3

The putative ERE at −56 to −43 is not a functional ERE. (A) Schematic of mutations of DBH promoter. (B) PC12 cells were co-transfected with ERα and β-gal expression vectors and either wild-type rDBH(−249/+21)/Luc or mutant rDBH(−249/+21)/Luc, mutated at the −56 ERE (−56ERE/Del, −56ERE/Right Mut or −56ERE/Left Mut). Cells were treated with 0 or 10 nM E2 for 24 h and assayed for luciferase activity. Luciferase activity was normalized to β-gal activity and is expressed relative to the respective untreated control taken as 1. * p≤0.05 compared to untreated control.