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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Neuroendocrinol. 2011 Feb;23(2):168–176. doi: 10.1111/j.1365-2826.2010.02086.x

Figure 4.

Figure 4

17 β-oestradiol induces increased rat DBH promoter activity and elevated mRNA levels within hours. (A) PC12 cells were transfected with ERα expression vector and treated with 10 nM E2 for 0, 6, 12, 24, or 48 h. Total RNA was isolated and analyzed by northern blot. DBH mRNA levels are normalized to 18S rRNA levels and expressed relative to the 0 h group and taken as 1. n = 4–5 replicates per group * p≤0.05 compared to 0 h control. (B) PC12 cells were co-transfected with ERα and β-gal expression vectors and rDBH(−200/+21)/Luc. Cells were treated with 0 or 10 nM E2 for 3, 6, 12 or 24 h. Luciferase activity was normalized to β-gal activity and is expressed relative to the respective untreated control taken as 1. * p≤0.05 compared to untreated control.