Cholesterol transport inhibitors inhibit Hh-induced Ptc internalization and en expression. a Cholesterol distribution in compound-treated salivary glands monitored by filipin staining. b Wild-type salivary gland cells show membrane-localized Ptc (green, left-most panel, top) and no en expression (green, left-most panel, bottom). Right panels, Ptc localization (top) and en expression (bottom) in compound-treated salivary glands misexpressing Hh. Nuclei are shown in red