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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: J Hepatol. 2010 Jul 24;53(5):797–804. doi: 10.1016/j.jhep.2010.05.022

Fig. 4. (A) GRP78 promoter activity in response to NS2 protein of different genotypes.

Fig. 4

All constructs were cloned in pcDNA3.1/Zeo+ with identical Kozak sequence and HA-tag. NS2 null mutant served as a control. Expression of NS2 protein of different genotypes is shown at the top, and actin served as a loading control. (B) Activation of GRP78 by inducible expression of NS2. Huh-7 cells stably transfected with the NS2 construct were transfected with the GRP78 promoter construct followed by addition of doxycycline (1 µg/ml). Luciferase activity (left panel) and GRP78 mRNA (middle panel) were measured two days later. Data are presented as mean ± SD (n-4). NS2 and GRP78 proteins were revealed by Western blot (right panel).