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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: J Hepatol. 2010 Jul 24;53(5):797–804. doi: 10.1016/j.jhep.2010.05.022

Fig. 5. Full-length HCV replicon induced severer ER stress than the subgenomic replicon.

Fig. 5

Huh-7.5 cells (~2.8 × 106) were electroporated with 4 µg of RNA of the FL or SG replicon (Con1 strain), and seeded into 24-well plates. A replication deficient FL replicon was included as a negative control. After an overnight incubation, cells were transfected, in quadruplicate, with the promoter constructs of GRP78, GADD153, ATF6, and CMV (50ng/well). (A) Luciferase assays at day 2 p.t. Relative luciferase activity (RLA) is presented as the mean ± SD (n=4) and adjusted by transfection efficiency. (B) Immunofluorescent staining and Western blot analysis of NS5B protein. Note that the signals represent the steady state protein produced from input replicon RNA and newly synthesized RNA.