Fig. 4.

LPS pretreatment reduces inflammatory factors following I/R injury. Serum concentrations of TNF-α, IL-1β, and IL-6 were measured before and after I/R injury in three experimental groups (n = 12 in each group). Before ischemia/reperfusion, concentrations of inflammatory factors (TNF-α, IL-1β, IL-6) from rats pretreated with LPS had moderately increased compared with untreated rats. However, after ischemia for 30 min and reperfusion for 3 h, serum from LPS + I/R group rats had decreased inflammatory factors concentrations dramatically compared with serum from I/R group rats. LPS pretreatment could induce the release of inflammatory factors moderately before I/R but reduce the release of inflammatory factors greatly in the following ischemia/reperfusion injury. As an inhibitor of HSP70, quercetin could incur higher level of inflammatory factors in Q-LPS + I/R group compared with that in LPS + I/R group. These results indicated that HSP70 induced by LPS pretreatment was responsible for the reduction of inflammatory factors following I/R injury. Data are means ± SEM. *P < 0.01 compared with indicated groups