Table 1.
Identification and subcellular localization of putative disulfide proteins
| No.a | Protein name (PP no.) | SC (%) | MS/MS (sequence)b | Subcellular localizationsc |
|---|---|---|---|---|
| 1 (5) | Elongation factor Tu (0440) | 20 | R.VQDPLEIVGLR.D | Cytoplasmic |
| 2 (7) | Succinate dehydrogenase catalytic subunit (4190) | 30 | R.LASLDDPFSVFR.C | |
| 3 (8) | Ribosomal protein L3 (0454) | 59 | R.LEEGDFQAGDLIK.A | |
| 4 (10) | Alkyl hydroperoxide reductase, C subunit (2439) | 19 | R.GTFVINPEGQIK.I | |
| *5 (10) | Antioxidant, AhpC/Tsa family (1084) | 53 | K.AYDVESEGGVAFR.G | |
| 6 (12) | Hypothetical protein (0425) | 19 | S.CEAFLEAER.A | |
| 7 (13, 14) | Sensory box protein (0216) | 17 | G.AVEDREYR.I | |
| 8 (15) | Hypothetical protein (2462) | 15 | S.AELLEQAR.K | |
| 9 (18) | Ribonucleotide-diphosphate reductase alpha subunit (1179) | 28 | K.LNAVSSGGDSAPVQAAGPAPVPK.A | |
| 10 (24) | Dihydrolipoamide dehydrogenase (4187) | 22 | R.RPVTTDLLASDSGVTIDER.G | |
| 11 (3, 4) | Hypothetical protein (1245) | 52 | R.VTPAVNLDELDVAASK.E | Cytoplasmic membrane |
| 12 (9) | Polyhydroxyalkanoate granule associated protein GA2 (5007) | 15 | K.TAAEKPAAKPAAKPAAAK.P | |
| 13 (11) | Thiol peroxidase (3587) | 28 | R.EFLENYGVAIADGPLAGLAAR.A | |
| 14 (22) | Hypothetical protein (3699) | 46 | R.GNVTELSEFEYGK.L | |
| 15 (1, 2) | Outer membrane protein OprF (2089) | 38 | P.SVGVGLNFGGSPK.Q | Outer membrane or periplasmic |
| 16 (6) | Basic amino acid ABC transporter (4486) | 48 | R.LDGTVADATLLEDGFLK.T | |
| 17 (8) | Basic amino acid ABC transporter (0282) | 25 | T.QENAYLDLVSGR.I | |
| 18 (15) | Hypothetical protein (0587) | 22 | R.VFQEQSTSFNLPPGTVDVR.L | |
| 19 (19) | Outer membrane protein, bacterial surface antigen family (1599) | 26 | K.TGFFQDIQLSR.D |
SC sequence coverage
aNumbers in parenthesis indicate the band number (Figure 1B, b)
bOnly a single representative peptide sequence is shown, even in cases where multiple peptide sequences were obtained
cPrediction of subcellular localization