Skip to main content
. 2011 Mar 11;12(4):350–357. doi: 10.1038/embor.2011.17

Figure 1.

Figure 1

Analysis of USP21 cross-reactivity against Ub, NEDD8 and ISG15. (A) USP21 and a catalytically inactive mutant USP21i were tested against Ub-AMC and NEDD8-AMC. Right panel: Michaelis–Menten kinetic analysis for Ub-AMC. Error bars represent s.d. from the mean. (B) USP21 activity against ISG15-AMC, as tested in (A). Right panel: Michaelis–Menten kinetic analysis for ISG15-AMC. (C) Kinetic parameters of USP21 cleavage of Ub-AMC and ISG15-AMC, derived from the plots in A and B. (D) Specificity analysis of USP21 against diUb of indicated linkages. Time course assays were resolved on 4–12% NuPAGE gels, followed by silver staining. ISG15, interferon-stimulated gene 15; NEDD8, neural precursor cell expressed, developmentally downregulated 8; PAGE, polyacrylamide gel electrophoresis; Ub, ubiquitin; USP, Ub-specific protease; WT, wild type.