Cancer cells are more sensitive to WRAP53 depletion compared with normal cells. (a) WB analysis of WRAP53 and β-actin levels in H1299 and HEK293 cells treated with siControl, siWRAP53 no.;1 and siWRAP53 no.;2 for 72 h or 48 h, respectively. (b) The bars show the percentage of active caspase 3-positive H1299 and HEK293 cells treated with siControl, siWRAP53 no.;1 and siWRAP53 no.;2 for 96 h. Error bars represent standard error of three independent experiments. (c) WB analysis of WRAP53 and β-actin levels in HDF, AG06814 and MCF10A cells treated with siControl, siWRAP53 no.;1 and siWRAP53 no.;2 for 72–96 h. (d) The bars show the percentage of active caspase 3-positive HDF, AG06814 and MCF10A cells treated with siControl, siWRAP53 no.;1 and siWRAP53 no.;2 for 96 h. Error bars represent standard error of three independent experiments