Figure 1.

In vitro synergism of TSA/C6-ceramide in cancer cells death. CaOV3 ovarian cancer cell (a) and L3.6 pancreatic cancer cell (c) were treated with different doses of TSA (0, 50, 100, 200 and 500 ng/ml) in the presence or absence of C6-ceramide (10 μg/ml) for 48 h, cell viability was detected by MTT assay as described, both cells were cultured in basic DMEM medium. CaOV3 (b) and L3.6 cells (d) were treated with varying doses of C6-ceramide (0, 2.5, 5, 10, 20, 40 and 60 μg/ml) in the presence or absence of TSA (100 ng/ml) for 48 h, cell viability was detected by MTT assay, both cells were cultured in basic DMEM medium. CaOV3 (e) and L3.6 cells (f), both maintained in 10% FBS in DMEM, were treated with combination of different dose of TSA and C6-ceramide, cell viability was detected by MTT assay. CaOV3 (g) or L3.6 cells (h) was exposed to HDACIs sodium butyrate (SB, 1 mM) or suberoylanilide hydroxamic acid (SAHA, 1.5 μM), in the presence or absence of C6-ceramide (10 μg/ml) for 48 h, after which the cell viability was determined by MTT assay. Data are presented as the means±S.D. for three independent experiments. Experiments in this figure were repeated at least three times and similar results were obtained. ^*P<0.05 versus same dose of TSA group without C6-ceramide treatment (ANOVA). ^#P<0.05 versus same dose of C6-ceramide group without TSA treatment (ANOVA)