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. 2011 Apr 14;7(4):e1001335. doi: 10.1371/journal.ppat.1001335

Figure 3. MoHYR1 complements the S. cerevisiae Δhyr1 mutant.

Figure 3

The yeast strains BY4741 (wild type) and BY4741 YIR037W (Δhyr1) were obtained from the ATCC. The mutant was complemented with the wild type copy of itself, the MoHYR1 gene, and the MoHYR1 containing mutations at each of the two cysteine residues (cys39Ala and cys88Ala). All strains were spotted onto YPD plates containing 0 mM, 2 mM and 4 mM hydrogen peroxide. Neither the YIR037W strain, nor the two cysteine residue mutants grow at the non-permissive concentration however the yeast mutant is partially rescued by the MoHYR1 copy. This experiment was repeated ten times with similar results.