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. 2011 Apr 14;7(4):e1001335. doi: 10.1371/journal.ppat.1001335

Figure 9. ROS scavenging in the plant rescued the hyr1 mutant phenotype.

Figure 9

(A) Conidia of Δhyr1 (B25) were mixed with 0.5 mM ascorbic acid and inoculated onto the leaf surface. Infected leaves were stained for ROS 24 hpi. (B) Conidia were mixed with water and inoculated onto the leaf surface. Leaves were first treated with 0.5 mM ascorbic acid for 1 hour and stained for ROS 24 hpi. (C) From left to right: Δhyr1 (B25), Δhyr1 (B33) (where susceptible barley leaves were treated with 0.5 mM ascorbic acid for 1 hour and then inoculated with mutant spores in water) ectopic (B40), ectopic (B60), wild type (70-15). Scale bar  = 20 µm for all confocal images.