Figure 5. ISG induction is limited to IE1 expressing cells.

TetR and TetR-IE1 cells were placed in the upper and lower chambers, respectively, of transwell dishes. Cells were growth-arrested and then treated in one of two ways. A) TetR-IE1 cells in the bottom chambers were treated with IFN-γ for 24 h or were left untreated (w/o). B) TetR cells in the upper and TetR-IE1 cells in the lower chambers were treated with doxycycline (Doxy) for 72 h or were left untreated (w/o). RNA was prepared from each compartment and analyzed by qRT-PCR with primers for the CXCL9, CXCL11, GBP4, and IE1 genes. Results were normalized to TUBB and mean values with standard deviations from two biological and two technical replicates are shown in comparison to untreated TetR-IE1 cells (set to 1).