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. 2011 Apr 14;7(4):e1002016. doi: 10.1371/journal.ppat.1002016

Figure 10. IE1 increases STAT1 occupancy at ISG promoters.

Figure 10

TetR and TetR-IE1 cells were treated with doxycycline for 72 h. During the last 30 min of doxycycline treatment TetR cells were incubated in the presence of solvent, IFN-γ or IFN-α. ChIP assays were carried out with polyclonal rabbit antibodies against STAT1 (A) or STAT2 (B). The fraction of immunoprecipitated DNA relative to input DNA was determined by qPCR with primers specific for the non-ISGs GAPDH (white circles), ribosomal protein L30 (RPL30) (black circles), and TUBB (gray circles) as well as for the ISGs GBP4 (white squares), CXCL9 (black squares), TAP1 (gray squares), IFIT2 (white triangles), and OAS1 (black triangles). Mean values of two technical replicates from TetR-IE1 cells (IE1) and from IFN-γ- or IFN-α-treated TetR cells are presented relative to solvent-treated TetR cells (set to 1). Results from five (A) or two (B) independent experiments are shown.