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. 2011 Apr 14;7(4):e1002020. doi: 10.1371/journal.ppat.1002020

Figure 5. Treatment with X4-ZFNs is effective in ccr5Δ32 homozgyous human CD4+ T cells.

Figure 5

(A) Ccr5Δ32 CD4+ T cells were stimulated on day 0 and transduced on day 1 with an Ad5/F35 vector expressing the X4-ZFNs, R5-ZFNs, or an untransduced control. On day 5, cells were HIV-infected with a mock, primary X4 HIV-1 (Bk132), lab-adapted X4 HIV-1 (HxB2), or a primary R5X4 HIV-1 (R3A). Live cells were counted approximately every two days. Cells were restimulated on day 13 (arrows). (B) Cxcr4 disruption frequency was assessed at various times by 454 deep sequencing. Disruption remained stable in the absence of HIV-1 infection, but profoundly increased in the presence of the three HIV-1 strains examined. Data shown is from one of two representative experiments.