Table 4. IgM confirmation detection of five cases of HRP-LD5 assay positive alone serum samples^*.

No. of individual serum	First collected samples						Second collected samples (after three weeks)
	Chang Zheng kit		HRP-LD5 based assay		anti-HCV IgM assay		Chang Zheng kit			HRP-LD5 based assay		anti-HCV IgM assay
	BME		BME		BME		BME			BME		BME
	−	+	−	+	−	+	−	+		−	+	−	+
1	0.110	0.078	0.433	0.054	0.216	0.048	0.503		0.258	1.354	0.372	1.121	0.053
2	0.178	0.060	0.358	0.047	0.187	0.064	0.280		0.263	0.957	0.675	0.565	0.042
3	0.021	0.045	0.465	0.049	0.312	0.071	0.287		0.117	1.255	0.136	1.010	0.039
4	0.142	0.076	0.876	0.068	0.550	0.055	0.803		0.426	1.876	0.463	1.233	0.064
5	0.090	0.053	0.332	0.032	0.228	0.051	0.652		0.289	1.479	0.244	0.980	0.057

*Serum samples treated without (−) or with (+) β-mercaptoethanol (BME) were used to test anti-HCV IgM antibodies. Data shown are the A₄₅₀ values, and the cut-off values of the ELISA were either 0.200 (HRP-LD5 based assay and established anti-HCV IgM assay) or 0.150 (Chang Zheng kit).