Figure 1. Exosomes isolated from M.tb infected cells inhibit IFN-γ induced surface expression of MHC class II and CD64 on BMMØ.

Exosomes were isolated from uninfected RAW264.7 cells (Un exo) or RAW264.7 cells infected with M.tb H37Rv (Rvexo). BMMØ were treated with exosomes for 18 hours or left untreated (RC) followed by +/− IFN-γ stimulation for an additional 18 hours. Cells were stained with either PE conjugated anti-MHC class II or anti-CD64 and analyzed by flow cytometry. Shown are the number of cells stained with MHC class II (A) or CD64 (B) and representative FACS plots for each treatment; MHC-II (C) and CD64 (D). Isotype control antibody was used to define background staining. Results are representative of three individual experiments plus standard deviations; asterisk (*) indicates a p value ≤0.05 between +/− IFN-γ treatments.