Table 1. Use of the HRM assay to analyze different regions of the HIV genome.
| Region analyzed | Gag1 | Gag2 | Pol |
| Corresponding region in HXB2* | 1998 - 2096 | 2071–2278 | 2373–2597 |
| Primer sequences | |||
| Forward | 5′-AAATTGCAGGGCCCCTAGGAA | 5′-ACTGAGAGACAGGCTAATTTTTTAG | 5′-AAATGGAAACCAAAAATGATAG |
| Reverse | 5′-TTTCCCTAAAAAATTAGCCTGTCT | 5′-GGTCGTTGCCAAAGAGTGATTTG | 5′-CATTCCTGGCTTTAATTTTACTG |
| HRM amplicon size | 99 bp | 208 bp | 225 bp |
| HRM scores for plasmidsa Median (range) | 3.8 (3.8, 3.8) | 3.4 (3.2, 3.8) | 3.5 (3.4, 3.5) |
| HRM scores for 6–8 week old infants (n = 31)b,c Median (range) | 4.7 (4.4, 7.3) | 4.2 (3.6, 6.3) | 4.1 (3.3, 6.7) |
| HRM scores for 12 month old infants (n = 17)c Median (range) | 5.2 (4.3, 7.1) | 4.6 (3.8, 6.4) | 4.3 (3.3, 5.9) |
| HRM scores for 18 month old infants (n = 15)c Median (range) | 5.1 (4.6, 6.3) | 4.9 (4.2, 7.8) | 4.4 (4.0, 5.9) |
a
Control reagents; two subtype A plasmids and two subtype D plasmids.
b
One infant did not have an HRM result for the Pol region.
c
These HRM scores can be compared to those obtained in an observational study of 79 children in Uganda [51] (median age 4.7 years, range 0.6–12.4 years). In that study, the median HRM score in the Gag2 region was 5.9 (range: 3.8–11.9).