Table 2. Loading rates and half-maximal loading time of HLA-DR1 variants loaded with HA306-318 as determined by fluorescence polarization.

HLA-DR1	rate [1/h]	t1/2 [h]
wt	0.06±0.01	12.2±3.0
αQ9A	0.54±0.05	1.3±0.1
αE11A	1.05±0.1	0.7±0.1
βR71A	0.07±0.01	15.5±1.6
βH81F	0.13±0.02	5.5±0.9
βN82A	3.83±0.66	0.2±0.03