Table 3. Melting points of empty HLA-DR1 variants as determined by thermal shift assay (TSA) and dynamic light scattering (DLS).
HLA-DR1 | Tm [°C] by TSA | Tm [°C] by DLS |
wt | 66.4±0.5 | 71.5 |
αQ9A | 52.2±0.3 | 55 |
αE11A | 51.8±0.2 | 55.3 |
βR71A | 62.9±0.8 | n.d.* |
βH81F | 62.5±0.4 | 65.5 |
βN82A | 51.3±0.1 | 51.1 |
*) not determined.