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. 2011 Apr 14;6(4):e18762. doi: 10.1371/journal.pone.0018762

Figure 1. Changes in flow cytometry cell cycle profiles for cells overexpressing NETs.

Figure 1

HEK293T cells expressing mRFP-NET fusions were recovered by trypsinization and analyzed by flow cytometry at 48 h post-transfection. Data were analyzed using FlowJo software and histogram overlays are displayed as %Max, scaling each curve to mode = 100%. The red line is the mRFP expressing cells in the population while the blue line is the untransfected cells in the population (the majority of cells were not transfected). The transfected and untransfected populations were both set on the scale to 100 for the 2N population so that increases or decreases in the 4N peak reveal changes in the cell distribution. Arrows indicate significant changes in cell cycle profile between transfected and non-transfected cells.