Figure 9. NET4/TMEM53 knockdown did not affect levels of other nuclear envelope proteins linked to the cell cycle or cyclins.

(A) Protein lysates were recovered from U2OS cells treated as in Figure 8 and reacted on Western blots with antibodies to the various proteins. This experiment was repeated 3 times and a representative Western blot is shown. (B) Cyclin levels were quantified from three separate experiments analyzed by LI-COR using fluorescent secondary antibodies and are plotted normalized to the actin control. Standard errors are shown.