Figure 2. N induces ROS production and caspase 3 activation in MCF7 and 293T cells.

(a) N and P expression in MCF-7 cells. 24 h after transfection, total RNA was extracted from the cells and RT-PCR was performed as described in methods. Gel pictures showing expression of N and P. (b) Analysis of ROS generation by DCF fluorescence as described in Methods. Representative fluorescent images and corresponding mean fluorescence intensity. (c) Determination of caspase 3 activation in MCF7 cells by FITC fluorescence as described in Methods. Results are expressed as the fold increase in fluorescence and are given as the mean ± SD for three experiments.