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. 2011 Apr 14;6(4):e18765. doi: 10.1371/journal.pone.0018765

Figure 5. Validation of 2DE by real time and semi quantitative RT-PCR.

Figure 5

24 h after transfection, total RNA was extracted from the cells and real time and semi quantitative RT-PCR was performed as described in methods. (a) Gel pictures showing differential expression of PARK7 and PHB. Fold change in expression was analyzed by software ImageJ. Data was normalized with beta actin as the control house keeping gene. (b) Relative quantification of GAPDH and MAP2K4 mRNA in N transfected and control MCF7 cells. The change in gene expression is expressed as fold change in relation to control. Results are presented as the mean ± SD from 3 different experiments.