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. 2011 Apr 14;6(4):e18842. doi: 10.1371/journal.pone.0018842

Figure 1. LGP2 deficiency decreases intracellular dsDNA signaling.

Figure 1

(a) MEFs from wild-type mice (+/+) and LGP2-deficient mice (−/−) were transfected with 5 µg/ml of poly(dA-dT) for the indicated amount of time and total RNA was isolated from cells. The relative abundance of IFNβ, CXCL10, and CCL5 mRNA was determined by quantitative RT-PCR. Error bars indicate standard deviation for duplicate PCR reactions. (b) MEFs or BMDM from wild-type mice (+/+) and LGP2-deficient mice (−/−) were transfected with 5 µg/ml poly(dA-dT) or (c) ISD. At indicated time points after transfection, media was collected and IFNβ levels were quantified by ELISA. Results are representative of at least 2 independent transfection experiments carried out in duplicate. Student’s t test was performed: *(p<0.05), **(p<0.01), and ***(p<0.005).