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. 2011 Apr 14;6(4):e18848. doi: 10.1371/journal.pone.0018848

Figure 1. Hsp90α, MMP-2 and several co-chaperones are present in the conditioned media of breast cancer cells.

Figure 1

Lysate and conditioned media were collected from MDA-MB-231 cells. 20 µg of protein was loaded into each lysate lane and 80 µg of protein was loaded into each conditioned media lane and immunoblotted for the Hsp90α, MMP-2, Hsp70, Hop, Hsp40, p23, Hip, and β-tubulin. The first six proteins were found to be present in both the cell lysate and in the conditioned media, while Hip was only found in the lysate. β-tubulin was used as a negative control. The molecular weight markers are indicated on the left.