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. 2011 Apr 14;6(4):e18848. doi: 10.1371/journal.pone.0018848

Figure 3. The presence of Hsp70, Hop, Hsp40, and p23 increase the interaction between Hsp90α and MMP-2.

Figure 3

Using recombinant proteins, two co-immunoprecipitations were performed, one with Hsp90α and MMP-2 alone and one with Hsp90α, MMP-2, Hsp70, Hop, Hsp40, and p23 present using the same quantities as in Fig. 2. Hsp90α and MMP-2 primary antibody were used to pull down Hsp90α and then the resulting proteins were run on an SDS-PAGE and blotted for Hsp90α. The bands were then subject to densitometry to determine the relative interaction between Hsp90α and MMP-2 with and without the presence of the co-chaperones. Experiments were repeated three times. P-value<0.001.