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. 2011 Apr 14;6(4):e18848. doi: 10.1371/journal.pone.0018848

Figure 4. Hsp90α and co-chaperones Hsp70, Hop, Hsp40, and p23 are sufficient to activate MMP-2 in vitro.

Figure 4

(a) 0.5 µg Pro-MMP-2 was incubated with and without 4 pg of activated MMP-2 and was incubated with 1 µg Hsp90α, 1 µg Hsp70, 0.25 µg Hop, 0.1 µg Hsp40, and 0.25 µg p23 recombinant proteins as indicated in the figure at 30°C for 30 minutes. 0.5 ug Pro-MMP-2 was activated in 1 mM APMA at 37°C for 2 hours (b) The same amounts of protein as above were incubated with 100 µM ATP, 100 µM ATPγS, or alone at 30°C for 30 minutes. The proteins were then added to a non-reducing sample buffer and then run on a gelatin containing SDS-PAGE. The gels were renatured for 40 minutes, digested for 18 hours and stained with Coomassie Brilliant Blue. Hsp90α Chaperone Complex = Hsp90α, MMP-2, Hsp70, Hop, Hsp40, and p23. Experiments were repeated three times. (a) P-value<0.05 (b) #P-value>0.3, *P-value<0.05.