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. 2011 Apr 14;6(4):e18848. doi: 10.1371/journal.pone.0018848

Figure 5. Conditioned media from cancer cells contains necessary components to activate MMP-2.

Figure 5

(a) Conditioned media was collected from MDA-MB-231 cells, samples were incubated with and without 0.75 µg pro-MMP-2 for 5 minutes and the relative amount of active MMP-2 was analyzed via SDS-PAGE. (b) Conditioned media was collected and then treated with 30 µM Methylene Blue. 0.75 µg recombinant pro-MMP-2 was added to treated and untreated conditioned media. Samples were incubated at room temperature for 5 minutes and the relative amount of active MMP-2 was analyzed via SDS-PAGE. The difference in overall band intensity was taken into account in the densitometry calculations. Experiments were repeated three times. (* P-value<0.01, #P-value<0.05).