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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Cancer Res. 2011 Jan 11;71(2):572–582. doi: 10.1158/0008-5472.CAN-10-2314

Figure 5. Nuclear Corepressor (NCoR) is required for basal and androgen induced expression of INPP4B.

Figure 5

A. LNCaP cells were transfected with either control noncoding (C) or NCoR specific siRNA. Cells were plated in full serum medium, harvested 24 hours later and expression of NCoR, AR, and β-actin were analyzed by Western blot. B. LNCaP cells were transfected as in A and plated in css medium and treated with either vehicle (ethanol (V)) or 1 nM R1881. Cells were harvested 24 hours post-treatment and relative PSA expression was determined by quantitative RT-PCR. C. Cells were transfected as in A, plated in css medium and treated with either vehicle (ethanol (V)) or 1 nM R1881 for 24 hours. RNA was extracted and analyzed for INPP4B and 18S expression by quantitative RT-PCR. D. LNCaP cells transfected in parallel with A were plated in full serum and treated with either vehicle (ethanol (V)) or 10 μM bicalutamide (bic) for 24 hours. RNA was extracted and analyzed for INPP4B and 18S expression by quantitative RT-PCR. Star indicates statistically significant difference in expression in NCoR siRNA treated compared to the noncoding control treated cells with p<0.05.