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FcγR-mediated Rap1 activation increases Mac-1 affinity in U937 cells. Flow cytometric analysis with CBRM1/5, an Ab that recognizes an activation epitope of Mac-1, was performed on (A) unstimulated and (B) FcγR-ligated U937 monocytes transfected with pcDNA3.1 or Rap1GAP as described in Materials and Methods. Histograms are representative of five separate experiments.
