Table 2.

The effect of sABs on the affinity of MBP for maltose.

	Kd maltose (μM)	ΔΔG maltose (Kcal/mol)a	ΔΔG closed-open (Kcal/mol) b
MBP-233C	67c
MBP-233C + MCS1	1.9c	−2.1	−2.2
MBP-233C + MCS2	9.6c	−1.1	−1.5
MBP-233C + MCS3	3.6c	−1.7	−1.7
MBP-233C + MCS4	2.1c	−2.0	−2.3
wt-MBP	0.63d
wt-MBP + MOS1	10.5d	+1.6	+1.4

^aΔG of maltose binding with sAB -ΔG of maltose binding without sAB

^bΔΔG values from Table 1

^cAffinity measured using fluorescence of Alexa 488-labeled MBP-233C mutant

^dAffinity measured using intrinsic tryptophan fluorescence of wt MBP