FIGURE 2.

Amino acid sequence alignment of the 18 human DENN domain proteins. The amino acid sequences of the DENN domains correspond to the sequences of the UniProt long isoforms listed in Table 1. Alignment was performed with ClustalW and manually modified using Jalview. Shaded boxes represent conserved residues, with darker shadings based on increasing percent identity. The dashed border for the N terminus of the DENN module is used to highlight the varying lengths of the linker regions between the uDENN and DENN modules, where some DENN modules begin at the start of the dashed line and others begin at the start of the solid line. Secondary structure predictions are also indicated along the gray dashed line below the alignment. β-Strands and α-helices are represented as black arrows and red rectangles, respectively.