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. 2011 Mar 2;286(16):13905–13913. doi: 10.1074/jbc.M110.195370

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — ABT-737 treatment causes Bax activation in platelets. A–C, platelets (1.0 × 10⁸) isolated from C57BL/6J mice were incubated with 10 μm ABT-737 or vehicle for 2 h at room temperature. A, Western blot of platelet lysates for the expression of Bax after immunoprecipitation (IP) using mouse antibody that specifically recognizes activated Bax (6A7) or mouse control IgG (active Bax exposes an amino-terminal epitope (amino acids 12–24) that is recognized by 6A7). B, Western blot for the expression of Bak, Bax, CoxIV (cytochrome c oxidase IV), and GAPDH after cellular fractionation of the platelet lysates. C, Western blot for the expression of Bax after incubation of the platelet lysates with or without protein cross-linkers (5 mm bismaleimidohexane and 5 mm bis(sulfosuccinimidyl) suberate). IB, immunoblot.