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. 2011 Feb 22;286(16):13954–13965. doi: 10.1074/jbc.M111.220103

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Characterization of extracellular deleted variants of EWI-2 protein. A, schematic representation of the extracellular deleted variants of FLAG-tagged EWI-2. B, deleted EWI-2 proteins were expressed in HEK-293T cells. Surface-biotinylated proteins were immunoprecipitated with anti-CD81, anti-CD9, or anti-FLAG mAbs. Proteins were separated by SDS-PAGE under nonreducing conditions, transferred to a nitrocellulose membrane, and detected using HRP-streptavidin. EWI-2, CD81, and CD9 proteins are endogenously expressed in HEK-293T cells. The cleavage of EWI-2 does not occur in HEK-293T cells. Endogenous EWI-2 is indicated by an asterisk.