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. 2011 Feb 22;286(16):13954–13965. doi: 10.1074/jbc.M111.220103

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Motifs in CD81 important for its interaction with EWI-2/EWI-2wint. A, schematic representation of the different CD81/CD82 chimeras. B, EWI-2/EWI-2wint (wt) and chimeric CD81 proteins were co-expressed in CHO cells, surface biotinylated, labeled with [³H]palmitic acid, or directly lysed in PBS/Brij97/EDTA. IP were performed with 5A6, TS82b, or M2 antibodies. Proteins were detected using streptavidin-HRP, autoradiography, or immunoblotting. Oligomers of CD81 are indicated by an asterisk. C, chimeric CD81 proteins were expressed in CHO cells, biotinylated, lysed in PBS/Brij97/EDTA, and precipitated with 5A6. Proteins were resolved by SDS-PAGE under reducing (bottom) or nonreducing (top) conditions and detected using streptavidin-HRP.