FIGURE 8.

Mutation of glycine-rich motifs in transmembrane domains of CD81. A, schematic representation of mutations of glycine-rich motifs in TM1, TM2, and TM3 of CD81. The first amino acid (G/A), in each motif was replaced by a leucine residue (L). B, mutated CD81 proteins were co-expressed with wild-type EWI-2/EWI-2wint proteins (wt), biotinylated, lysed in PBS/Brij97/EDTA, and immunoprecipitated with 5A6 or M2 antibodies. Proteins were separated by SDS-PAGE under nonreducing conditions and detected using HRP-streptavidin.