FIGURE 1.

Functional importance of OA-NO₂-reactive Cys residues. A and B, HEK-293T cells were co-transfected with ARE-luciferase reporter vector, Nrf2-overexpressing vector, and vector expressing wild type Keap1 or Cys to Ser mutations of the indicated amino acids. 24 h after transfection, cells were treated with OA-NO₂, 15d-PGJ₂, or SFN for 16 h, and luciferase activity was measured. Results are normalized to β-galactosidase and represented as -fold change versus pGL3-SV40-control vector for each treatment. Values are represented as mean ± S.D. (error bars). *, p < 0.05; **, p < 0.01 when compared with ARE-luc + Nrf2 + Keap1-wt.