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. 2011 Feb 5;286(16):14065–14072. doi: 10.1074/jbc.M110.182592

FIGURE 3.

FIGURE 3.

Hydrolysis of GA2 and Gb4Cer by recombinant NgaP. TLC shows the hydrolysis of GA2 and Gb4Cer. Aliquots of 5 nmol of GA2 or Gb4Cer were incubated with 10 milliunits of the enzyme at 37 °C for 30 min or 6 h in 20 μl of 25 mm sodium acetate buffer (pH 6.0) containing 0.2% detergent. The hydrolysis of GA2 or Gb4Cer was examined as described under “Experimental Procedures.” Lane 1, GA2 marker; lane 2, NgaP + GA2; lane 3, NgaP + GA2 + 0.2% taurodeoxycholate (TDC); lane 4, NgaP + GA2 + 0.2% Triton X-100; lane 5, LacCer marker; lane 6, Gb4Cer marker; lane 7, NgaP + Gb4Cer; lane 8, NgaP + Gb4Cer + 0.2% taurodeoxycholate; lane 9, NgaP + Gb4Cer + 0.2% Triton X-100; lane 10, Gb3Cer marker.