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. 2011 Feb 28;286(16):14080–14089. doi: 10.1074/jbc.M111.222323

FIGURE 4.

FIGURE 4.

Effect of the CT of tubulin and ion strength on the interaction between the α2B-AR CT and tubulin. A, effect of removing the CT of tubulin on its interaction with the α2B-AR CT. GST and GST-α2B-AR CT were incubated with 1 μg of purified tubulin or tubulin S. B, effect of increasing concentration of NaCl on the interaction between the α2B-AR CT and tubulin. GST and GST-CT were incubated with 1 μg of purified tubulin with increasing concentrations of NaCl (0–400 mm) in the reaction buffer. Upper panel, bound tubulin was analyzed by Western blotting using α-tubulin antibodies; lower panel, graphical representation of the inhibition of GST-α2B-AR CT interaction with tubulin caused by increasing concentrations of NaCl. The data shown are percentages of the bound tubulin in the absence of NaCl in the reaction buffer and presented as the mean ± S.E. of three experiments. *, p < 0.05 versus bound tubulin in the absence of NaCl.